Crop plant transgenes
Transgene Source Destination taxon Transformation method Promoter Terminator Transgene description Phenotype References Contact
5-UGT Solanum tuberosum Solanum tubesrosum var. Desiree Leaf disc, Agrobacterium tumefaciens, PGV2260 B33 tuber specific ocs 5-UGT gene (glucosyltransferase) Increased stability of anthocyanin; Increased pathogen resistance and higher fertility of the plant. Increased resistance against pathogen infection (Ervinia carotovora subsp. carotovora (Ecc)) unpublished szopa@ibmb.uni.wroc.pl
14-3-3 repression Solanum tuberosum Solanum tuberosum var. Desiree Leaf disc, Agrobacterium tumefaciens, PGV2260 35S CaMV ocs Repression of all 14-3-3 protein isoforms in plants. Increased activity of nitrate reductase, SPS, SS; Increase in sucrose, starch and protein levels. Decreased levels of catecholamines, lipid, flavonoids and phenolic acids. Weakened antioxidant properties. 2003. Plant Science 165:731-741 szopa@ibmb.uni.wroc.pl
pHB Ralstonia eutropha Linum usitatissimum var. Nike Hypocotyl, Agrobacterium tumefaciens, PGV2260 35S CaMV (phbA), 16R 14-3-3 potato isoform promoter (phbA+C) nos (phbA), ocs (phbA+C) Genes encoding pHB synthesis enzymes (phbA - beta-ketothiolase, phbC - polihydroxybutyrate synthase) Synthesis of new pHB polymer; Increased resistance to Fusarium oxysporum and Fusarium culmorum infection; decrease in starch and glucose content; Increased production of seeds 2004. J Biotechnol 107:41-54 szopa@ibmb.uni.wroc.pl
Tyr-Dec Petroselium crispum Solanum tuberosum var. Desiree Leaf disc, Agrobacterium tumefaciens, PGV2260 35S CaMV ocs Tyr-Dec encoding gene (Eli5) Increase of catecholamines content; decrease of starch content in tubers, combined with increased soluble sugars content; Increased levels of phenolic compounds (especially chlorogenic acid and isochlorogenic acid (correlated with increased antioxidant activity); Increased resistance of tubers to pathogen infection (Ervinia carotovora subsp. carotovora (Ecc)) 2004 PPB 42: 103-109 szopa@ibmb.uni.wroc.pl
DFR Petunia hybrida Solanum tuberosum var. Desiree Leaf disc, Agrobacterium tumefaciens, PGV2260 35S CaMV ocs DFR (dihydroflavonol reductase, sense position) Increased levels of antocyans and phenolic acids; significant increase of antioxidative potential 2004 J Agric Food Chem 52(6): 1526-33 szopa@ibmb.uni.wroc.pl
W92 Petunia hybrida Solanum tuberosum var. Desiree Leaf disc, Agrobacterium tumefaciens, PGV2260 35S CaMV ocs CHS (chalcone synthase), CHI (chalconate isomerase), DFR (dihydroflavonol reductase); all in sense position Increased content of anthocyans and phenolic acids; No change in anti-oxidative potential 2004 J Agric Food Chem 52(6): 1526-33 szopa@ibmb.uni.wroc.pl
TuMV coat protein Brassica napus var. oleifera cv. Westar Brassica napus var. oleifera cv. Westar Agrobacterium tumefaciens, strain LBA4404, plasmid pJR1Ri 35S CaMV nos TuMV coat protein (CP; GenBank Accession: X65978) gene in a good translation context (AACA) and with a start codon (ATG) Resistance to TuMV (turnip mosaic virus) 2003 Mol Breed 11: 83-94 pleh@igr.poznan.pl
Download full set of data on IBMB (Wroclaw University) transgenes (including metabolome) (Excel 97)
Contact: szopa@ibmb.uni.wroc.pl